Check the Weight and balance as well. Set to Medium.
Once you are lined up, set the Throttle to about 50% to start with, when N1 settles at about 60-70% then slowly increase throttle to about 90%. (Flaps 2)
If you are using 28L, with a Southerly wind, it will be coming from your right hand side which will try and push your tail to the LEFT and your nose to the right. So gently use the rudder to counteract this as you build up speed. V1 should be about 130-135 with rotate around 140-145kts, when you reach this speed gently pull the nose up only about 5 degrees (first line on the HUD) and you should slowly take off, continue to use the rudder to help keep a straight line. Soon after you will be able to retract gear and activate the HD Autopilot, then SP Autopilot (set to about 220kts). slowly increase your nose up till you have a VS of about 3000 ft/m.
Once above about 1000ft bring the flaps up and maybe lower the nose so you have VS around 2500 ft/m. Set the ALT section of your AP and then slowly increase speed to about 250kts till above 10000 ft.